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Incorporated of [1,2-13C2] acetate and [Me-13C]-methionine into the mycotoxin terretonin, a metabolite of Aspergillus terreus, announce its accumulation by a alloyed polyketide–terpenoid pathway.
The genomic arrangement abstracts from several Aspergillus breed has accurate to be a benefaction to advisers absorbed in belief the analysis and biochemistry basal accessory metabolism in this important fungal genus.
Incorporation of 13C-labelled acetates and methionine, 14C and 2H-labelled ethyl 3,5-dimethylorsellinate into terretonin by cultures of Aspergillus terreus adumbrated that its biosynthesis gain via a alloyed polyketide-terpenoid (meroterpenoid) pathway. Incorporation of 18O2 gas and ethyl 3,5-dimethylorsellinate  doubly labelled with 13C and 18O in the carbonyl of the carboxyl accumulation and at the C-6 position into terretonin and ascertainment of 18O isotope-induced accouterment in the 13C n.m.r. spectra and GC/MS studies of the accomplished metabolites bent the agent of all of the oxygen atoms in and provided mechanistic acumen into the biosynthetic pathway.
Complete assignments of all the resonances in the 1H and 13C n.m.r. spectra of terretonin accept been fabricated application a array of apparent and two-dimensional alternation methods. Nuclear Overhauser enhancements and spin-spin couplings empiric in the 1H n.m.r. spectrum announce that the atom adopts a anatomy in band-aid agnate to that empiric in the clear structure.
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