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Chemical properties: 1 - Bromo - 2 - nitrobenzene as buff acicular crystal. 43 ℃ melting point, boiling point 261 ℃, relative density 1.6245 (80/4 ℃). Soluble in ethanol, soluble in ether and benzene, insoluble in water.
Purpose: 1 intermediates in organic synthesis. Two pharmaceutical intermediates, also can be in EPA8081A method analysis of organochlorine pesticides in water and soil to internal standard.
Production methods: the adjacent nitroaniline by the following steps. Neighbouring nitroaniline and a mixture of sulfuric acid cooling to 0 to 5 ℃, slowly add sodium nitrite solution under stirring, potassium iodide to the reaction of starch test paper turned blue. Will the diazonium salt solution under stirring slowly add 40 ℃ of cuprous bromide solution. After cooling, were extracted with ether extract with alkali, hydrochloric acid solution and water washing, a quick product after recovery of ethyl ether. The yield of about 70%.
Category: toxic substances
Flammability hazard characteristics: flame burning; Heat release toxic bromide, nitrogen gas
Storage and transportation characteristics: warehouse ventilated dry at low temperature; And oxidant, food additives
Agent: water fog, foam, carbon dioxide, sandy soil

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Amphotericin B has a carboxyl and amino structure, both between acid and alkali. Polyene antibiotic is mainly used for deep fungal infection, such antibiotics combined with fungal cells sterol, damage of the membrane permeability, causing fungi in potassium ion leakage, nucleotides, amino acids thereof, destroy the normal metabolism and its antibacterial effect. Cells lack of sterol except mycoplasma bacteria cannot be polyene antibiotic effect. Free sterol and sterol competition on the cell membrane polyene antibiotic, and make the polyene antibiotic effect reduced.

Amphotericin B for multiple alkene antifungal antibiotics, by influencing the cell membrane permeability of play to inhibit the growth of fungi. Used in treatment of severe deep fungus or systemic infection caused by the internal organs.

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English name: octylphenoxypolyethoxyethanol
English referred to as: Nonidet P - 40 (sometimes called NP - 40, but should with another kind of surfactant NP - 40 (nonylphenoxypolyethoxyethanol) separate.
Chinese name: ethyl phenyl polyoxyethylene. Commonly used non-ionic sex than degeneration detergent and surface active agent [1].
Similar products: IGEPAL CA - 630 (Sigma - Aldrich)


Nonidet P - 40 (octylphenoxypolyethoxyethanol; ethyl phenyl polyoxyethylene) solution is designed to used for cracking, melting, stable protein and electrophoresis of non ion detergent and surface active agent. Often used in cell cracking, protein processing, immunohistochemical processing, hybrid washing, etc. The product is to use, aseptic filtration, seedless enzyme pollution, stable performance, high purity is even and the repeatability is good, simplified operation.

Reagent characteristics

Ethyl phenyl polyoxyethylene (Nonidet P - 40; nonaethylene glycol octylphenyl ether) for no ion of detergent, the Critical Micelle Concentration (Critical Micelle Concentration) low, and protein binding force strong, used to prevent material molecular hydrophobic interactions, and ensure that protein fully dissolved and structure stability. Especially used for membrane protein denaturation not under the condition of dissolved.

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