English name: octylphenoxypolyethoxyethanol
English referred to as: Nonidet P - 40 (sometimes called NP - 40, but should with another kind of surfactant NP - 40 (nonylphenoxypolyethoxyethanol) separate.
Chinese name: ethyl phenyl polyoxyethylene. Commonly used non-ionic sex than degeneration detergent and surface active agent [1].
Similar products: IGEPAL CA - 630 (Sigma - Aldrich)
Uses
Nonidet P - 40 (octylphenoxypolyethoxyethanol; ethyl phenyl polyoxyethylene) solution is designed to used for cracking, melting, stable protein and electrophoresis of non ion detergent and surface active agent. Often used in cell cracking, protein processing, immunohistochemical processing, hybrid washing, etc. The product is to use, aseptic filtration, seedless enzyme pollution, stable performance, high purity is even and the repeatability is good, simplified operation.
Reagent characteristics
Ethyl phenyl polyoxyethylene (Nonidet P - 40; nonaethylene glycol octylphenyl ether) for no ion of detergent, the Critical Micelle Concentration (Critical Micelle Concentration) low, and protein binding force strong, used to prevent material molecular hydrophobic interactions, and ensure that protein fully dissolved and structure stability. Especially used for membrane protein denaturation not under the condition of dissolved.
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