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Take the Pig Bile Powder is about 0. 1 g, precision said, adding 10% sodium hydroxide solution 5 ml, 120 ℃ heat 4 hours, put the cold, and hydrochloric acid to adjust the pH to 2 ~ 3, shake well. With ethyl acetate extract four times, each time 10 ml, the extracted liquid, dry, residual ethanol is dissolved, transfer to 10 ml volumetric flask, adding ethanol to scale, shake well, as the test solution. Other precision according to take in the phosphorus pentoxide decompression drying in the dryer for 24 hours of hyodeoxycholic acid reference substance right amount, adding ethanol made from each 1 ml solution containing 1 mg, as a reference substance solution. According to thin layer chromatography (appendix Ⅵ B) test, precision draw 1 mu l of test sample solution and reference substance solution 1 and 3 mu mu l l, intersection, respectively, in the same silica G thin layer plate, with a new configuration of isooctane ether - glacial acetic acid - n-butyl alcohol - water - the upper solution (10:5:5:3:1) as developing agent, swing, remove and dry spray with 10% ethanol solution of sulfuric acid in the 105 ℃ heating to the spot color is clear, take out, on the thin layer plate covering the same size glass plate, fixed with tape around as thin layer chromatography (TLC) (appendix Ⅵ B, thin layer scanning method) scan, wavelength: lambda s = 375 nm, lambda R = 650 nm, measuring the absorbance of the integral value and reference substance absorbance integral value, calculation, quick.

This article calculated on dry goods, including hyodeoxycholic acid (C24H40O4) shall not be less than 16. 0%.

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Probiotics,Best Probiotics, Probiotics Price ,Natural Probiotic Probiotics,Best Probiotics, Probiotics Price ,Natural Probiotic